Typhoid IgG/IgM Rapid Test Cassette(S/P)
SUMMARY
Typhoid fever is caused by S. typhi, a Gram-negative bacterium. World-wide an estimated 17 million cases and 600,000 associated deaths occur annually1. Patients who are infected with HIV are at significantly increased risk of clinical infection with S.typhi2 . Evidence of h. pylori infection also presents an increase risk of acquiring typhoid fever. 1-5% of patients become chronic carrier harboring S. typhi in the gallbladder. The clinical diagnosis of typhoid fever depends on the isolation of S. typhi from blood, bone marrow or a specific anatomic lesion in the facilities that cannot afford to perform this complicated and time consuming procedure, Widal Test (also referred as WeilFelix Test) is used to facilitate the diagnosis. However, many limitations lead to difficulties in the interpretation of the Widal test.
In contrast, the Typhoid Rapid Test Cassette is a simple and rapid laboratory test. The test simultaneously detects and differentiates the IgG and the IgM antibodies to S.typhi specific antigen in serum or plasma thus aid in the determination of current or previous exposure the S.typhi.
DIRECTIONS FOR USE
a. Bring the pouch to room temperature before opening it. Remove the test cassette from the sealed pouch and use it as soon as possible. Best results will be obtained if the assay is performed within one hour.
b. Place the test cassette on a clean and level surface. Hold the dropper vertically and transfer 1 drop of serum or plasma (40 ul) to the specimen well of the test cassette, then add 1 drop of buffer (40 ul) * and start the timer. Avoid trapping air bubbles in the specimen well. See the illustration below.
c. Wait for the colored line(s) to appear. Read the result at 15 minutes, do not interpret the result after 20 minutes.
* NOTE: It should be add one more drop of (approx. 40ul) buffer to the sample well of the test cassette if the specimen runs too slowlyTo use a micropipette: Pipette and dispense 10 µl of whole blood to the specimen well of the test
cassette, then add 1 drop of buffer (approximately 40 µl) and start the timer.
3. Wait for the colored line(s) to appear. The test result should be read at 10 minutes. Do not interpret the result after 20 minutes.
